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. Author manuscript; available in PMC: 2013 Jul 19.
Published in final edited form as: Biochem J. 2011 Apr 15;435(2):381–390. doi: 10.1042/BJ20101752

Figure 3. Graded transfections of V5–nSMase3 in HEK-293, MCF-7 and A549 cells.

Figure 3

HEK-293, MCF-7 and A549 cells were transfected as indicated with V5–nSMase3 for 24 h. Cell lysates were analysed for V5–nSMase3 expression by immunoblotting. Blots shown are representative of at least four immunoblots for each cell line.