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. Author manuscript; available in PMC: 2013 Jul 19.
Published in final edited form as: Biochemistry. 2003 Dec 2;42(47):13929–13940. doi: 10.1021/bi034843p

Figure 1.

Figure 1

Immunoblotting analyses of iPLA2β-immunoreactive protein expression in 832/13 INS-1 cells. Aliquots (50 μg) of cytosol prepared from INS-1 cells transfected with either an empty vector (V) or vector containing iPLA2β cDNA construct (OE) and from 832/13 INS-1 cells were analyzed by SDS–PAGE, and the proteins were transferred onto immobolin-P PVDF membrane. The electroblot was then processed for immunoblotting analyses, and immunoreactive iPLA2β protein was visualized by ECL.