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. Author manuscript; available in PMC: 2013 Jul 19.
Published in final edited form as: Biochemistry. 2003 Dec 2;42(47):13929–13940. doi: 10.1021/bi034843p

Figure 10.

Figure 10

iPLA2β-immunoreactive protein expression in pancreatic islets and iPLA2β message in islets and 832/13 INS-1 cells. (A) Immunoblotting analyses. Aliquots (10–50 μg) of cytosolic protein prepared from rat pancreatic islets were analyzed by SDS–PAGE, and the proteins were transferred onto immobolin-P PVDF membrane. The electroblot was then processed for immunoblotting analyses, and iPLA2β-immunoreactive protein was visualized by ECL. (OE, iPLA2β-overexpressing INS-1 cells.) (B) Comparison of iPLA2β message in islets and 832/13 cells. The deduced message sequence of islet iPLA2β (7) is illustrated along with the sequence coverage of the PCR products generated using five overlapping primer sets and the 832/13 INS-1 cell cDNA as template. (PCR1–5, sequences of products generated using primer sets 1–5 described in the Materials and Methods, respectively.)