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. Author manuscript; available in PMC: 2013 Jul 19.
Published in final edited form as: Biochemistry. 2003 Dec 2;42(47):13929–13940. doi: 10.1021/bi034843p

Figure 7.

Figure 7

ESI/MS analyses of glycerophosphocholine (GPC) lipid species in 832/13 INS-1 cells supplemented with arachidonic acid in the absence or presence of BEL. 832/13-INS-1 cells were treated with either vehicle alone (A), with arachidonic acid for 6 h (B) or 24 h (C), or pretreated with BEL (25 μM) prior to arachidonic acid supplementation for 24 h (D). Lipids were extracted and analyzed as Li+ adducts by ESI/MS/MS in positive ion mode by scanning for precursor ions that undergo neutral loss of 183. This loss reflects the elimination of phosphocholine headgroup and identifies GPC lipids (18, 36).