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. Author manuscript; available in PMC: 2014 Jun 20.
Published in final edited form as: Cell. 2013 Jun 20;153(7):1494–1509. doi: 10.1016/j.cell.2013.05.026

Figure 2. Comparison of the accessibility of the N-termini of Syt1 and of the E-Syts to the extracellular space.

Figure 2

(A) Confocal images of live HeLa cells expressing Syt1-EGFP and mRFP-Sec61β showing the predominant PM localization of Syt1 in these cells. Bottom images: high power view of the region outlined by dotted lines in the merge field. (B–E) Fluorescent images of HeLa cells co-expressing mRFP-Sec61β and Myc-Syt1 or Myc-E-Syts as indicated, and incubated with anti-Myc antibodies. The Myc epitope was accessible in the case of Myc-Syt1 but not in the case of Myc-E-Syts. (F) Fluorescent images of SH-SY5Y neuroblastoma cells co-expressing mRFP-Sec61β (red) with either Myc-Syt1 or Myc-E-Syts (green) and incubated with anti-Myc antibodies before fixation (left panels, “surface”) or after fixation and permeabilization (right panel, “permeabilized”). See also Figure S1.