Fig 1.

Analysis of an influenza A virus 2009/H1N1 strain with point mutations in the matrix gene by real-time PCR (A) and agarose gel electrophoresis (B). The positive control (line and lane 1), patient culture isolate (lines and lanes 2 and 3), and negative control (line and lane 4) were tested by the Prodesse ProFlu+ real-time PCR assay. The PCR product was analyzed in real time for fluorescent output (A) and by agarose gel electrophoresis with ethidium bromide staining (B). Lanes L, molecular weight ladders.