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. 2013 Jun;51(6):1861–1867. doi: 10.1128/JCM.03069-12

Table 2.

Relevant phenotypic and genotypic test results for the 703 strains studied

Reference ID (no. of isolates)a Vitek MS ID Optochin CO2b BS testc Pneumococcal capsule identification PCR result
No. of isolates with sequencing
No. of isolates (%) with Vitek MS
psaA MFP ply lytA recA sodA 16S rRNA Correct ID Incorrect ID Unresolved ID No ID
Pneumococcal isolates (334) 331 (99.1) 3 (0.9)
    S. pneumoniae (292) S. pneumoniae S ND Yes 292
    S. pneumoniae (37) S. pneumoniae S Soluble No or NDd 37
    S. pneumoniae (1) S. pneumoniae R (R) Soluble 3 + + + + 1 1
    S. pneumoniae (1) S. pneumoniae R (R) Soluble 23F + + 1 1
    S. pneumoniae (1) S. mitis/S. oralis S (S) Not soluble 23 + + + + 1 1
    S. pneumoniae (1) S. mitis/S. oralis S (S) Not soluble 37 + + 1 1
    S. pneumoniae (1) S. mitis/S. oralis S (S) Not soluble ND + + 1 1
Nonpneumococcal isolates (369) 335 (90.8) 9 (2.4) 21 (5.7) 4 (1.1)
    Other mitis group streptococci (166) 155 (93.4) 5 (3) 5 (3) 1 (0.6)
        S. pseudopneumoniae (1) S. pseudopneumoniae R (S) Not soluble No ND + + 1 1
        S. pseudopneumoniae (1) S. pseudopneumoniae R (S) Not soluble ND ND + + 1 1
        S. pseudopneumoniae (1) S. pseudopneumoniae R (S) Not soluble ND ND 1 1
        S. mitis/S. oralis (126)e S. mitis/S. oralis R 126
        S. gordonii (7) S. gordonii R 4 7
        S. cristatus (1) S. cristatus R 1 1
        S. sanguinis (7) S. sanguinis R 3 7
        S. parasanguinis (11) S. parasanguinis R 1 11
        S. australis (3)f S. parasanguinis R 3 3
        S. australis (2)f S. sanguinis R 2 2
        S. massiliensis (1)f No ID R 1 1
        S. oralis/S. parasanguinis (1) S. parasanguinis R 1 1
        S. mitis group (4) 2 S. mitis/oralis, 1 S. parasanguinis, 1 no ID R Not soluble 4 4
    Non-mitis group streptococci (184) 166 (90.2) 4 (2.2) 14 (7.6)
        S. salivarius group (39) 39 (100)
            S. salivarius subsp. salivarius (35) S. salivarius 2 35
            S. vestibularis (4) S. vestibularis 3 4
        S. mutans group (2) 2 (100)
            S. mutans (2) S. mutans 2
        S. anginosus group (111) 101 (91) 1 (0.9) 9 (8.1)
            S. constellatus (37) S. constellatus 2 37
            S. anginosus (48) S. anginosus 5 48
            S. constellatus/S. anginosus (5) S. constellatus 5 5
            S. constellatus/S. anginosus (4) S. anginosus 4 4
            S. anginosus (1) S. constellatus 1 1
            S. intermedius (16) S. intermedius 16
        S. bovis group (32) 24 (75) 3 (9.4) 5 (15.6)
            S. gallolyticus subsp. gallolyticus (4) S. gallolyticus subsp. gallolyticus 4
            S. gallolyticus subsp. pasteurianus (18) S. gallolyticus subsp. pasteurianus 18
            S. gallolyticus subsp. pasteurianus (2) S. gallolyticus subsp. gallolyticus 2 2
            S. bovis (1) S. bovis 1
            S. lutetiensis/S. bovis/S. equinus (1) S. bovis 1 1
            S. lutetiensis/S. bovis/S. equinus (4) S lutetiensis 4 4
            S. lutetiensis/S. bovis/S. equinus (1) S. gallolyticus subsp. gallolyticus 1 1
            S. infantarius subsp. infantarius (1) S. infantarius subsp. infantarius 1 1
    Related genera (19) 14 (73.7) 2 (10.5) 3 (15.8)
        Abiotrophia defectiva (1) A. defectiva 1
        Granulicatella adiacens (1) G. adiacens 1
        “Granulicatella para-adiacens”f/G. adiacens/G. elegans (1) No ID 1 1
        Gemella morbillorum/G. haemolysans (1) G. morbillorum/G hemolysans 1 1
        Helcococcus kunzii (2) Helcococcus kunzii 2
        Facklamia tabacinasalis (1)f No ID 1 1
        Facklamia ignava (1)f No ID 1 1
        Aerococcus viridans (2) A. viridans 2
        Aerococcus urinae (8) A. urinae 8
        Aerococcus sanguinicola (1)f No ID 1 1
Total (703) 8 51 5 666 (94.7) 12 (1.7) 21 (3) 4 (0.6)
a

BS test, desoxycholate solubility test.

b

Reference identifications were obtained using Rapid ID32 Strep strips or the VITEK2 system by default, or with gene sequencing when stated.

c

S, susceptible; R, resistant. Results were obtained under an atmopshere of increased CO2; parentheses indicate a result obtained under an ambient atmosphere.

d

Including three polyagglutinable isolates and 12 nonagglutinable isolates. ND, not determined.

e

Including 52 S. oralis, 68 S. mitis, and 6 S. mitis/S. oralis isolates.

f

Species absent from the Vitek MS database.