Agarose gel electrophoresis of PCR products. Species, variety and major genotype are depicted in brackets. Lanes: M, DL2000 ladder; 1, WM148 (C. neoformans var. grubii VNI); 2, Bt60 (C. neoformans var. grubii VNB); 3, WM626 (C. neoformans var. grubii VNII); 4, WM628 (C. neoformans AD hybrid VNIII); 5, WM629 (C. neoformans var. neoformans VNIV); 6, WM179 (C. gattii VGI); 7, WM178 (C. gattii VGII); 8, WM161 (C. gattii VGIII); 9, WM779 (C. gattii VGIV); 10, CBS10488 (C. neoformans×C. gattii BD hybrid); 11, CBS10496 (C. neoformans × C. gattii AB hybrid); 12, negative control.