Table 1.
Precision and accuracy of HIV-1 and HIV-2 detection for different viral loads and with different lanes of the biosensor chipsa
| HIV type | Load (TCID50s)b | CV (%)c |
Mean ± SD | Overall mean ± SD | |
|---|---|---|---|---|---|
| Lane 1 | Lane 2 | ||||
| HIV-1 | 672 | 9.8 | 10.6 | 10.2 ± 0.6 | 16.4 ± 5.7 |
| 480 | 16.4 | 22.8 | 19.6 ± 4.5 | ||
| 224 | 15.7 | 22.9 | 19.3 ± 5.1 | ||
| HIV-2 | 406 | 10.7 | 17.1 | 13.9 ± 4.5 | 15.8 ± 2.9 |
| 289 | 19.3 | 16.7 | 18.0 ± 1.8 | ||
| 160 | 14.9 | 16.1 | 15.5 ± 0.8 | ||
a
Data were obtained from 5 chips (each with 2 lanes coated with HIV-1 or HIV-2 antibodies and 2 lanes coated with control antibodies). All measurements were taken at 5 min after the addition of virus.
b
The viral loads shown for HIV-1 are equivalent to 189,010, 135,007, and 63,003 viral particles, respectively.
c
CV (%), the percent coefficient of variation, is defined as the standard deviation divided by the mean for all phase shifts (Δφ) measured.