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NV-N-R5-1 phage binding to NV VLPs in NoV-negative stool suspension. NV VLPs (0 to 50 ng) (x axis) were diluted in 10% NoV-negative stool suspension and captured by immobilized NV polyclonal antibody. NV VLPs were detected by 1 × 109 PFU NV-N-R5-1 phage, a 1:2,500 dilution of NV polyclonal antibody, or a 1:500 dilution of MAb 3912. Binding was detected by M13 antibody or secondary antibody and determination of the OD450 (y axis).
