Fig 2.
PCR restriction enzyme analysis (PRA) of the 441-bp Telenti fragment of the hsp65 gene. The first five lanes include digests with BstEII (fragments of 235-115-100), and lanes 8 to 12 include the same isolates digested with HaeIII (fragments of 145-125-55). The digest patterns for M. marseillense, M. chimaera, and M. intracellulare are indistinguishable. Lanes 1 and 8, M. marseillense, household water; lanes 2 and 9, M. chimaera, household water; lanes 3 and 10, M. intracellulare, patient isolates; lanes 4 and 11, M. chimaera type strain DSM 44623T; lanes 5 and 12, M. intracellulare type strain ATCC 13950T; lane 6, 100-bp ladder (100, 200, 300, 400); lane 7, pGEM ladder (51, 65, 75, 126, 179, 222, 350). Molecular weight standard sizes are shown to the left and the right.