Fig 2.
Growth curves of T. brucei cultures grown in nutrient-depleted medium. (A) Equivalent growth observed for HMI11 (diamonds) compared to diluted HMI11 without vitamins (Dil-Vit; triangles) or diluted HMI11 without amino acids and nucleotide precursors (Dil-AA/N; squares). (B) Equivalent growth observed in HMI11 (triangles), basal medium with cysteine with glutamine (CQ; squares), or cysteine with phenylalanine and tryptophan (CFW; diamonds) but not in glutamine with phenylalanine and tryptophan (QFW; crosses). (C) Equivalent growth observed in HMI11 and CMM. (D) Short-term growth curves obtained with HMI11 and CMM showing a maximal cell density of around 5 × 106 cells ml−1 and subsequent cell death.