Table 1.

MIC of PlySs2 for various Gram-positive species^a

Species	Strain	MIC (μg/ml)
		Visual	Colorimetric
L. monocytogenes	HER 1184	8	16
	HER 1083	8	16
S. aureus	MSSA 8325	16	16
	MRSA MW2	16	32
	LyrA	32	32
	VISA III	32	64
GrAS	SF370	128	128
	MGAS 5005	128	256
GBS	090R	256	256
	Type II	512	512
E. coli	TOP10	>1,024	>1,024

^aAll MICs were evaluated visually for bacterial growth and with alamarBlue vital dye (colorimetrically) at concentrations from 0.5 to 1,024 μg/ml of PlySs2 for each strain of each species listed. There was a low MIC for MRSA MW2, as expected, and a higher MIC for GrAS strain MGAS 5005. The MIC of PlySs2 for the negative control, E. coli, was above the limits of the assay. For a reference, ClyS, LysK, and CHAP_K lysins have registered MICs against S. aureus strains from 30 to 80 μg/ml.