Table 2.
Treatment and concn (ng/ml)a | Tail DNA (%)b | Tail extent (μm)c | Tail olive moment (μm)d | RDIe |
---|---|---|---|---|
Negative control | 34.54 ± 1.05f | 58.08 ± 6.45 | 1,098.20 ± 18.01 | 0.00 |
Positive control | 66.38 ± 4.49 | 137.4 ± 29.32 | 3,521.06 ± 490.69 | 1.00 |
Colistin | ||||
550 | 39.08 ± 1.36 | 69.29 ± 0.08 | 1,289.91 ± 53.01 | 0.13 |
1,100 | 40.07 ± 5.99 | 71.44 ± 3.45 | 1,351.49 ± 33.52 | 0.17 |
4,400 | 45.46 ± 3.96 | 78.24 ± 4.22 | 1,600.21 ± 266.63 | 0.26 |
Each peptide was used at 2 ng/ml.
Tail DNA (%) = 100 − Head DNA (%).
The tail extent is the distance of DNA migration from the body of the nuclear core, and it is used to evaluate the extent of DNA damage.
Olive tail moment = (Tail mean − Head mean) × Tail % DNA/100.
RDI = (mean tail length of treated cells − mean tail length of negative control)/(mean tail length of positive control − mean tail length of negative control). MMS was used as a positive control, and untreated cells were used as a negative control.
Mean value ± standard derivation for 100 randomly selected cells (average ± standard deviation, n = 100).