Fig 3.

Crystal structure of pneumococcal MurA1. (A) Overall structure of S. pneumoniae MurA1_D39 in an open conformation. Ala₃₆₄ is represented as capped sticks and colored red. (B) Overall structure of S. pneumoniae MurA1_Hungary19A in a closed conformation, showing a citrate and a PEG molecule in the catalytic pocket (black-capped sticks). Thr₃₆₄ is represented as capped sticks and colored red. (C) Stereo view of the superposition of domain I of MurA1_D39 (orange) and MurA1_Hungary19A (blue). The Ala₃₆₄Thr mutation is represented in sticks and highlighted (star). Side chains of residues around position 364 are drawn as lines to show that there are no relevant modifications upon mutation. Dom., domain.