Fig 1.

SEVI binds bacteria in a charge-dependent manner. Fluorescently labeled S. aureus (A), E. coli (B), or N. gonorrhoeae (C) cells were preincubated with 35 μg/ml of biotinylated SEVI or SEVIala fibrils. SEVI:bacteria aggregates were then captured using antibiotin magnetic beads. The fluorescence intensity of the captured aggregates is shown. Error bars represent the standard errors of the means (SEM) from three to eight independent experiments. Data were analyzed with an exact Wilcoxon rank sum test. *, P = 0.05; ns, not significant.