Fig 4.

SEVI enhances phagocytic uptake of live bacteria by primary monocyte-derived macrophages and neutrophils (PMNs). Mid-log-phase bacteria were preincubated with SEVI or PBS and then added to MDM (A to C) or PMNs (D). After 1 h, inocula were removed and the MDM or PMNs were treated with gentamicin to kill extracellular bacteria. Viable intracellular bacteria were then counted by plating of serially diluted cell lysates. The results are plotted in terms of relative CFU compared to the CFU of cells exposed to bacteria alone (defined as 1). Error bars represent the SEM from three experiments, each performed with cells from different donors. Data were analyzed with a Wilcoxon signed rank test. *, P = 0.05.