Fig 5.

SEVI-mediated enhancement of S. aureus phagocytosis by macrophages is unaffected by staphylococcal protein A. A spa-deleted mutant of the UAMS1 strain of S. aureus (KPL16) and its genetically complemented, protein A-positive counterpart (21) were incubated with MDM in the presence or absence of SEVI, and bacterial pathogenesis was measured by gentamicin resistance assay (see Fig. 4 legend). Data are presented as the fold increase in bacterial phagocytosis in the presence of SEVI compared to that of cells exposed to bacteria alone (defined as 1). Error bars represent the SEM from three experiments, each performed with cells from different donors.