Fig 6.

SEVI enhances binding of killed bacterial particles to human macrophages. Killed E. coli (left panels) or S. aureus (right panels) cells labeled with FITC were mixed with SEVI fibrils or buffer alone and then incubated with MDM pretreated with cytochalasin D. After 40 min, cells were harvested and flow cytometric analysis was performed. The median fluorescence intensity (MFI) of MDM incubated with fluorescent bacteria is shown. Error bars represent the SEM from three independent experiments, performed with MDM from different donors in each case. Data were analyzed with an exact Wilcoxon rank sum test. *, P = 0.05.