Figure 2.
Analysis of TCR-transduced T cells in vivo. (A) P14 splenocytes were stimulated in vitro with gp33 peptide and transduced with the indicated 3D TCR construct, restimulated with splenocytes pulsed with the WT1 peptide after 7 days, and analyzed by flow cytometry on day 5 postrestimulation. (B and C) 10 × 106 TCR-transduced P14 T cells were transferred into irradiated B6 mice along with 10 × 106 irradiated B6 splenocytes pulsed with the WT1 peptide. Blood from treated mice (solid line) was analyzed by flow cytometry on day 7 after T-cell transfer for relative size (FSC) and CD25 expression compared with a Thy1.1+ control (shaded histogram).