| Column | Beckman Ultrasphere C18 (5 μm), 4.6 mm × 250 mm |
| Guard column | C18 (7 μm), 15 mm × 3.2 mm |
| Flow rate | 1.8 ml/min |
| Run time | 35 min |
| Injection volume | 20 μl |
| PDA detection wavelength | 325 nm |
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