Table 1. Diversity measures (number of haplotypes and of segregating sites, θ_w and π) of the three genomes in S. nutans and S. otites and results of the neutrality tests (Tajima's D between π and θ_w and Fu and Li's D with S. latifolia as an outgroup).

Genome	Genes	Species	Length (pb)	Pop/seq	Number of haplotypes	Segregating sites	θw ±s.d.( × 10−3)	π±s.d.( × 10−3)	Tajima's D	Fu and Li's D (S. latifolia)
Nuclear	X4	S. nutans	578	22/44	4	23	9.15±1.08	4.70±2.05	−1.6062	1.7216
		S. otites	578	22/44	4	6	2.39±1.15	4.66±0.46	2.4906*	1.1846
	ELF	S. nutans	210	27/54	10	6	6.27±2.99	6.45±0.86	−0.3085	−0.4465
		S. otites	210	27/54	10	7	7.31±3.31	10.27±0.78	1.0516	1.2367
	ATUB	S. nutans	389	19/38	38	42	25.70±8.36	45.08±1.60	0.9779	0.8380
		S. otites	389	19/38	38	39	23.36±7.82	41.51±1.46	0.8380	1.0604*
	ABCtrp	S. nutans	352	35/70	5	6	3.54±1.66	1.90±0.36	−1.1045	1.1483
		S. otites	352	35/70	5	6	3.54±1.66	3.59±0.44	0.0325	1.1483
Mitochondrial	Cob	S. nutans	980	26/26	11	9	2.41± 1.07	2.21±0.33	−0.2663	1.4610
		S. otites	980	26/26	4	4	1.07±0.61	1.97±0.20	2.2611*	1.0941
	Cox1	S. nutans	1037	22/22	16	18	4.76±1.89	3.76±1.89	−0.7760	−1.0939
		S. otites	1037	22/22	8	9	2.38±1.08	2.74±0.28	0.5095	1.4774
Chloroplast	GS	S. nutans	533	37/37	7	7	3.75±1.74	2.75±0.17	−0.7533	0.4829
		S. otites	533	37/37	3	5	2.88±1.49	5.57±0.49	2.2884	0.9282
	psbA	S. nutans	299	37/37	9	12	10.69±4.29	13.73±0.80	0.8813	0.5275
		S. otites	299	37/37	3	9	8.52±3.68	10.86±2.61	0.4298	0.9282
	LF	S. nutans	505	37/37	6	6	3.27±1.60	1.74±0.38	−1.4607	0.2110
		S. otites	505	37/37	3	10	5.61±2.36	8.85±1.11	1.7480	1.4035
	matK	S. nutans	684	37/37	7	6	2.10±1.03	3.2±0.22	1.4091	1.0488
		S. otites	684	37/37	3	4	1.40±0.78	1.94±0.45	0.9297	0.9282
Chloroplast concatenated	Cp	S. nutans	2021	37/37	11	31	4.04±1.37	4.28±0.31	0.1400	0.6943
		S. otites	2021	37/37	6	28	3.77±1.29	5.71±0.76	1.7257	1.6834

*P<0.05.