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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Aging Cell. 2013 Apr;12(2):303–311. doi: 10.1111/acel.12055

Figure 1. Age-associated B cells (ABC) accumulate in spleen and bone marrow of aged C57BL/6 mice.

Figure 1

Panel A: Spleen and bone marrow were obtained from young (2 mo.) and old (26 mo.) C57BL/6 mice, processed, and stained for follicular B cells (FO; IgM+ CD21/35+ CD23+ CD19+), age-associated B cells (ABC; IgM+ CD21/35− CD23− CD19+), and marginal zone B cells (MZ; IgM+ CD21/35high CD23− CD19+). In the analyses shown, immature B cells (AA4.1+) and B1 B cells (CD43/S7+) were gated out. Data are shown as the percentage of these B cell populations within the total mature, non-B1 pool. Panel B: Cumulative results showing the percentages and numbers of ABC and FO B cells in spleens and of ABC and FO-like, recirculating mature B cells in bone marrow of aged and young mice. Results shown are for 22 young mice (2-5 mo), 12 aged mice at 24 mo, 5 aged mice at 25-26 mo, and 8 aged mice at 27-29 mo of age. Total spleen cells averaged 124 × 106 in young mice and 112 × 106 in old mice; total bone marrow cells averaged 48 × 106 for young and 55 × 106 for aged mice for combined femur/tibia pairs. Statistical significance as shown: *, p<0.045, **, p<0.0083; ***, p<0.0009; ****, p<0.0001.