Fig. 4.

Mutant frequency at microsatellite repeats is significantly increased in rAML. (A) Spontaneous mutant frequency measured at six non-coding polyadenine microsatellites increases with age in spleen cells of unirradiated animals (Pearson’s, P < 0.0001). A linear fit is shown. (B) Non-clonal mutant frequency is increased greater than 2-fold in AML cells compared to control spleen cells from age-matched animals (*, t test P < 0.001). No persistent increase in mutant frequency was detected in normal spleen cells of irradiated animals compared with those from unirradiated age-matched controls. Error bars indicate standard error.