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To assess the concentration dependency of 2-ACP thiol protection, graded NAPQI concentrations (2–128μM) were incubated (15 minutes) with different concentrations of 2-ACP (25–200 μM). At the end of the preincubation period, GSH (30 μM) was added, and sulfhydryl content was measured after 15 minutes by the DTNB method. Data are expressed as mean percent control ± S.E.M. (n = 3–4 experiments). Calculated IC50 values are provided in parentheses.
