Fig. 1.

Agonist-induced calcium mobilization in DA receptor–transfected cells. HEK293T cells were transiently transfected with D₁R, D_2LR, D_2SR, D₁R + D_2LR, or D₁R + D_2SR, as indicated and described in Materials and Methods. Twenty-four hours later, cells were plated in 384-well plates and assayed the following day for calcium mobilization after stimulation by DA (D₁R + D_2LR EC₅₀ = 73.8 nM, D₁R + D_2SR, EC₅₀ = 58.2 nM). Data are representative of three independent experiments done with the same assay conditions on different days. Data are expressed as percentage of control, normalized to the maximum signal seen via DA stimulation of D₁R + D_2LR transfected cells. Error bars indicate S.E.M. from multiple wells within the representative experiment.