Fig. 3.

Stimulation of D₁R + D_2LR–mediated calcium mobilization by either D₁R- or D₂R-selective agonists. HEK293T cells were transfected with D₁ R+ D_2LR as described, plated 24 hours later in 384-well plates, and assayed for calcium accumulation the following day. Cells were stimulated with one of the following agonists as indicated: DA, the D₁R-selective agonist SKF83822, the D₂R-selective agonist quinpirole, or both SKF83822 and quinpirole (D₁R + D_2LR EC₅₀ = 610.8 nM) combined. Control cells expressing the D₁R, D_2SR, or D_2LR individually did not show a significant calcium response to concurrent agonist administration. Data are expressed as a percentage of control maximum DA-stimulated response and are representative of two independent experiments performed with the same assay conditions on different days. Error bars indicate S.E.M. from multiple wells within the representative experiment.