Fig. 4.

Overexpression of βARKct blunts persistent AVP-induced P-ERK1/2 and abrogates AVP-mediated H9c2 cell survival. (A) Overexpression of βARKct (versus β-gal) significantly blunted the ERK1/2 phosphorylation response (from 10 to 60 minutes) following AVP stimulation. (B) The averaged data of (A). *P < 0.05; **P < 0.01 versus the same time point (unpaired t test). Overexpression of βARKct significantly blocked the AVP-dependent decrease in caspase 3/7 activity (C) and increase in cell survival (D). *P < 0.05 versus vehicle (phosphate-buffered saline; PBS)-stimulated β-gal control (one-way analysis of variance). All data are presented as the mean ± S.E.M. from three independent experiments. RLU, relative luminescence units; T-ERK1/2, total ERK1/2.