Table I.
Steady-state kinetic parameters for protein degradation and ATP hydrolysis by E.coli Lon. The error is that of non-linear-least-squares fitting. ATP per substrate was calculated by dividing Vmax for ATP hydrolysis by Vmax for protein degradation
| Substrate | Proteolysis Vmax (min−1 Lon6−1) | Proteolysis apparent KM (µM) | Proteolysis Hill constant | ATPase Vmax (min−1 Lon6−1) | ATPase apparent KM (µM) | ATPase Hill constant | ATP per substrate |
|---|---|---|---|---|---|---|---|
| cp6-sul20 | 3.6 ± 0.2 | 13 ± 2 | 1.2 ± 0.1 | 217 ± 16 | 9.3 ± 1.3 | 1.2 ± 0.1 | 60 |
| β20-cp6 | 2.3 ± 0.3 | 21 ± 6 | 1.2 ± 0.2 | 143 ± 5 | 2.6 ± 0.2 | 1.7 ± 0.1 | 62 |
| cp7-sul20 | 0.70 ± 0.03 | 2.8 ± 0.3 | 1.6 ± 0.3 | 130 ± 22 | 5.2 ± 2.4 | 0.9 ± 0.2 | 186 |
| β20-cp7 | 1.2 ± 0.1 | 8 ± 1 | 1.6 ± 0.3 | 130 ± 14 | 4.8 ± 1.0 | 1.3 ± 0.2 | 108 |
| cp6-Δ8-sul20 | 3.3 ± 0.1 | 13 ± 1 | 1.4 ± 0.1 | 215 ± 50 | 6.0 ± 3.0 | 1.1 ± 0.3 | 65 |