Table 1.
Fragment Ions Identified in the Tandem Mass Spectra of the Cross-linked Peptide
| Fragments from Peptide α (456 – 478) | Fragments from Peptide β (644 – 665) | ||||
|---|---|---|---|---|---|
|
| |||||
| Ion Type | Charge State | m/z | Ion Type | Charge State | m/z |
| b3α | 1 | 376.1868 | b4β | 1 | 412.2192 |
| b4α | 1 | 491.2147 | b6β | 1 | 608.3403 |
| b5α | 1 | 677.2937 | b7β | 1 | 709.3886 |
| b6α | 1 | 776.3622 | b10β | 4 | 912.4721 |
| b7α | 1 | 847.3981 | y11β | 2 | 672.8599 |
| b8α | 1 | 904.4179 | y12β | 2 | 722.3905 |
| b9α | 1 | 1005.4678 | y13β | 2 | 779.9047 |
| y3α | 1 | 371.2044 | y14β | 2 | 829.4113 |
| y4α | 1 | 484.2885 | y16β | 4 | 1121.3323 |
| y5α | 1 | 597.3713 | y16β | 5 | 897.2646 |
| y6α | 1 | 668.4098 | y18β | 4 | 1170.3571 |
| y8α | 1 | 852.5314 | y18β | 5 | 936.4869 |
| y9α | 1 | 965.6143 | y19β | 4 | 1195.1229 |
| y11α | 1 | 1135.7209 | y20β | 5 | 981.9154 |
| y12α | 1 | 1192.7418 | |||
| y12α | 2 | 596.8738 | |||
| y13α | 1 | 1293.7811 | |||
| y13α | 2 | 647.3988 | |||
| y14α | 5 | 817.8368 | |||
| y16α | 4 | 1061.5631 | |||
| y17α | 4 | 1079.3209 | |||
| y17α | 5 | 863.6592 | |||
| y18α | 4 | 1104.0878 | |||
| y18α | 5 | 883.4738 | |||
| y19α | 4 | 1150.6109 | |||
| y19α | 5 | 920.6881 | |||
| y20α | 4 | 1179.6131 | |||
| y20α | 5 | 943.6931 | |||
| y21α | 5 | 973.1121 | |||
| y21α | 6 | 811.0901 | |||
Peptide α: 456DLFDWVAGTSTGGILALAILHSK478
Peptide β: 644SPQVPVTCVDVFRPSNPWELAK665