Cholera toxin subunit B (CTB) injections into medial amygdala (MeA). A. Left series of images show representative injection into the MeA (HIT), and right series of images show representative missed injection aimed at MeA (MISS, dark label reflects CTB-conjugated Alexa 555); cytoarchitecture labeled with fluorescent Nissl (488) and fluorescent labels have been inverted and gray-scaled. Red outlined box is an enlarged representative image of injection. B. Overlay of region of largest cross-sectional extent of CTB label in each case in which MeA was successfully targeted (colors indicate individual successful injections). C. Average total volume of MeA encompassed by CTB injection and rostrocaudal distribution of CTB spread across MeA for each case. Abbreviations: Pir Piriform cortex, BLA Basal lateral amygdala, PLCo Posterolateral cortical amygdaloid area, ACo Anterior cortical amygdaloid area, MePV Medial amygdala posteroventral part, MePD Medial amygdala posterodorsal part, opt optic tract.