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. Author manuscript; available in PMC: 2013 Jul 19.
Published in final edited form as: Neuroscience. 2010 May 7;169(2):751–757. doi: 10.1016/j.neuroscience.2010.04.060

Figure 2.

Figure 2

Autoradiography showing antagonist reversal of agonist [35S]GTPγS binding by kappa opioid receptor. The brain sections were incubated with 2 mM GDP before adding 0.04 nM radiolabeled [35S]GTPγS and 10 µM U-69,593 with (a) or without 1 µM nor-BNI (b) and in presence of 0.04 nM radiolabeled [35S]GTPγS with 10µM U-69593 and 10µM unlabeled GTPγS (c). (Bregma = −4.80). 14C scale bar on the left indicates lower levels of G protein activation correspond to 33.1 Kbq/g and higher levels of G protein activation correspond to 1.1 Kbq/g.