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. Author manuscript; available in PMC: 2013 Jul 19.
Published in final edited form as: Neuroscience. 2010 May 7;169(2):751–757. doi: 10.1016/j.neuroscience.2010.04.060

Figure 4.

Figure 4

Digital images obtained from autoradiography of coronal brain sections showing the distribution of [35S]GTPγS binding stimulated by kappa opioid receptor. Autoradiograms represent brain sections incubated in presence of 0.04nM radiolabeled [35S]GTPγS without (basal) [a, d] or with 10µM U-69593 (specific binding) [b, e] and in presence of 0.04 nM radiolabeled [35S]GTPγS with 10µM U-69593 and 10µM unlabeled GTPγS (non specific binding) [c, f] in rat withdrawn for 1 day from 14 days of escalating-dose binge saline administration (a, b and c) and withdrawn for 1 day from 14 days of escalating-dose binge cocaine administration (d, e and f) (Bregma = −4.80).

14C scale bar on the left indicates lower levels of G protein activation correspond to 33.1 Kbq/g and higher levels of G protein activation correspond to 1.1 Kbq/g.