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RTL Effector Proteins Localize in the Plant Cell Nucleus.
RTL1, RTL2, and RTL3 were fused in frame to green fluorescent protein, respectively, to generate RTL:GFP and were transiently expressed in tobacco leaves via Agrobacterium-mediated delivery. Two days post agroinfiltration, confocal laser scanning microscopy was used to analyze samples. 4′,6′-Diamidino-2-phenylindole (DAPI) staining indicates nuclei. Scale bars = 30 μm. RTL:GFP fusion proteins localize in the plant cell nucleus.
