Figure 6.

Determining the DNA-Binding Specificities of Ralstonia RVDs.

The RVDs sequences of effectors, dHax3.RR.ND₁₂, dHax3.RR.HN₁₂, dHax3.RR.NP₁₂, and dHax3.RR.NT₁₂, and DNA sequences of EBE boxes in mBs3 or mBs4 promoter that drive uidA reporter expression are shown. Clones of the RTL effectors and EBE-containing mBs3 were co-argoinfiltrated into N. benthamiana leaves, respectively, as shown. dHax3.RR.ND₁₂ strongly and specifically activated the expression of polyC, preceded by T, -mBs3-driven uidA reporter gene (A, B), but not polyCdT-Bs3 (B). dHax3.RR.HN₁₂ showed a weak activation to A and G (C); dHax3.RR.NP₁₂ showed moderate interaction and activation of C, A, and G (D); and dHax3.RR.NT₁₂ showed weak binding and activation of T, A, G, and C (E).