Figure 2. Ccdc124 is present at the centrosome and it concentrates at the midbody in late stages of cytokinesis.

(A) HeLa cells were arrested at G2/M phase by sequential double thymidine and nocodazole treatments, then the drug was washed-off, cells were analyzed by immunofluorescence at time points with intervals of 15 minutes, and they were classified according to phases of mitosis, and cytokinesis. Samples of cells were then costained with anti-Ccdc124 and anti-γ-tubulin antibodies, and in interphase and prophase, Ccdc124 was observed as puncta in cells, and it is located in the MTOC area. In metaphase and anaphase cells, Ccdc124 appeared at the spindle poles. Ccdc124 was present at the midzone and at the spindle poles in late anaphase, and concentrated in the midbody during cytokinesis. (B) HeLa cells were transfected with the N-ter flag-Ccdc124 vector construct as in Figure 1. 48 hours later samples of cells were subjected to immunofluorescence costainings using anti-flag and anti-γ-tubulin Abs together. Representative micrographs of cells at different stages of cell cycle are given. Bars represent 10 µm.