Figure 3. Phenotypes associated with lifespan extension and stress resistance are observed in hyl-1;lagr-1.

(A) Pumping rates of N2, hyl-1;lagr-1, and an eat-2 mutant. The latter displays a pronounced reduction in pumping rate and is commonly used as a genetic model for dietary restricted animals. Bars represent the mean number of pumps per minute. Compared to N2 displaying a mean pumping rate of 201±2 pumps/min, hyl-1;lagr-1 shows a 17.4% decrease with a mean pumping rate of 166±2, P<0.0001, while eat-2 shows a 67.2% decrease with a mean pumping rate of 66±3, P<0.0001. The data represents an mean ± SEM of 15 measurements in 5 worms of each genotype. (B) Quantification of fluorescent beads in the pharynx and the anterior part of the intestine following a feeding period of 30 minutes. Compared to N2, hyl-1;lagr-1 displays 59% less fluorescence, P = 0.0026. Mean ± SEM is shown, n indicates the number of worms. (C) Mean total brood size of N2 and hyl-1;lagr-1. Compared to N2 which displays a mean brood size of 304±9, hyl-1;lagr-1 shows a 33% decrease with a mean brood size of 204±6, P<0.0001. Mean ± SEM is shown, n = number of worms examined. (D) Survival curves of N2 and hyl-1;lagr-1 subjected to heat-shock at 37°C. Compared to N2, hyl-1;lagr-1 shows increased resistance, P = 0.0016. A total of 60 worms of each strain were assayed. Mean ± SD of 3 experiments is shown. N2-worms (6) and hyl-1;lagr-1 worms (22) were censored but are incorporated in the analysis until the time they were censored. (E) Bars represent mean median heat shock survival from the 3 experiments shown in D. Compared to N2 which has a median survival of 7 hours, hyl-1;lagr-1 displays a 29% increase in heat shock resistance with a median survival of 9 hours. Error bars represent ± SD.