Figure 6. Binding of affinity purified anti-NY-ESO-1 antibodies to soluble DC-SIGN. Selected samples (based on material availability) were applied to wells coated with soluble DC-SIGN (sDC-SIGN). Bound IgGs were estimated by comparing the titration of samples on fixed amounts of DC-SIGN, and interpolated from a standard curve generated from IgGs that were directly bound to microwells. Lavender boxes = intact affinity purified IgGs; purple boxes = Sambucus nigra agglutinin (SNA)-lectin depleted IgG (SNA⁻); yellow boxes = SNA-enriched fraction of IgG (SNA⁺). Error bars represent standard deviations as obtained from hextuplicate determinations. Representative data from one out of two independent assays are reported.