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. 2013 Jul 19;8(7):e69066. doi: 10.1371/journal.pone.0069066

Figure 5. Transcription levels of hsp27 mRNA and αB-crystallin mRNA in myocardial cells after heat stress.

Figure 5

qPCR Ct values for hsp27 and αB-crystallin were normalized against β-actin. Expression of this housekeeping gene did not change in response to heat stress. The results showed that the transcription levels of hsp27 mRNA increased immediately and significantly (P<0.01) from 10 min after the onset of heat shock until 480 min, peaking at 360 min. The transcription levels of αB-crystallin mRNA also showed a significant (P<0.01) increase from 10 min after exposure to heat stress until 480 min, and peaked at 360 min. For αB-crystallin mRNA, a P<0.05; a*, P<0.01. For hsp27 mRNA, b P<0.01.