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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: FEBS J. 2013 Jun 18;280(15):3669–3684. doi: 10.1111/febs.12360

Figure 2.

Figure 2

Representative ITC isotherms at 25°C for the binding of ZRE duplex to the DB_WT domain of EGR1 at pH 5 (a), pH 6 (b), pH 7 (c) and pH 8 (d). The upper panels show raw ITC data expressed as change in thermal power with respect to time over the period of titration. In the lower panels, change in molar heat is expressed as a function of molar ratio of ZRE duplex to DB domain. The solid lines in the lower panels show the fit of data to a one-site model, as embodied in Eq [1], using Microcal Origin software.