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Dependence of [1/Kd] on pH for the binding of ZRE duplex to DB_WT (black), DB_H382A (red), DB-H382K (green), DB_H382R (blue), DB_H382E (magenta), DB_HH (brown) domains of EGR1 at 25°C. The error bars were calculated from at least three independent measurements to one standard deviation.