Table 1.
Antibodies used in immunohistochemical analysis of cervicovaginal tissues.
| Primary cell target | Antibody target | Source | Clone | Concentration (mg/ml) |
|---|---|---|---|---|
| T lymphocytes | CD3 | Dako A0452 | Pc | 1.0 |
| T lymphocytes | CD4 | Vector VP-C318 | 1F6 | ND (1:40) |
| T lymphocytes | CD8 | Vector VP-C325 | 1A5 | ND (1:50) |
| B lymphocytes | CD20 | Dako M0755 | L26 | 1.0 |
| Neutrophils | Myeloperoxidase | NeoMarker RB-373 | Rabbit polyclonal | ND (1:200) |
| Dendritic cells | CD207 | Abcam ab49730 | 12D6 | 10.0 |
| Natural killer cells | CD56 | Invitrogen 07-5603 | 12363 | ND (1:500) |
| Macrophages | CD68 | Dako M0814 | KP1 | 1.0 |
| Antigen presenting cells | HLA-DR | Novocastra NCL-LN3 | LN3 | 0.25 |
| Proliferating cells | Ki-67 | Dako M7240 | MIB1 | 1.0 |
| Papillomavirus-infected cells | HPV16 E6 | Santa Cruz SC-460 | C1P5 | 4.0 |
ND (ratio) = Concentration of antibody not specified by the manufacturer; antibody dilution is indicated.