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. Author manuscript; available in PMC: 2014 Aug 1.

Published in final edited form as: J Neurochem. 2013 Jun 11;126(3):331–337. doi: 10.1111/jnc.12308

(A) Schematic diagram of putative miRNA binding sites within the Oxt gene. Each box represents exons and UTRs. Also shown are the predicted duplex formation between miR-24 and the targeted Oxt gene and the comparisons of conserved targeting regions between mouse and other species (non-conserved regions are shown in red). (B) Luciferase activity of the Oxt cDNA reporter gene in the presence of the miRNA oligos. COS-7 cells were cotransfected with both the reporter gene and miRNA mimic oligos; miR-24, miR-379, miR-380, miR-540, miR-16, miR-34c, miR-338-3p and miR-744. Renilla luciferase data were normalized to firefly luciferase data and the fold value was compared to control vector transfection. Data show the means from three independent transfections (error bars indicate standard deviations; *, p≤0.05 for between control vector and miR-24).

(A) Schematic diagram of putative miRNA binding sites within the Oxt gene. Each box represents exons and UTRs. Also shown are the predicted duplex formation between miR-24 and the targeted Oxt gene and the comparisons of conserved targeting regions between mouse and other species (non-conserved regions are shown in red). (B) Luciferase activity of the Oxt cDNA reporter gene in the presence of the miRNA oligos. COS-7 cells were cotransfected with both the reporter gene and miRNA mimic oligos; miR-24, miR-379, miR-380, miR-540, miR-16, miR-34c, miR-338-3p and miR-744. Renilla luciferase data were normalized to firefly luciferase data and the fold value was compared to control vector transfection. Data show the means from three independent transfections (error bars indicate standard deviations; *, p≤0.05 for between control vector and miR-24).