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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Neurochem. 2013 Jun 11;126(3):331–337. doi: 10.1111/jnc.12308

Figure 4. Translational and transcriptional repression of mouse Oxt by miR-24.

Figure 4

Figure 4

(A) ELISA assay showed Oxt peptide expression was specifically reduced following transfection of the miR-24. Data show the means from three independent experiments (error bars indicate standard deviations; **, p≤0.001 between control vector and miR-24 and *; p≤0.05 between miR-24 and siControl). (B) qRT-PCR of Oxt after Oxt cDNA transfection with or without miRNA-transfection (miR-24 or siControl) into AtT-20 cells. (Inserted box) Visualized final qRT-PCR products. Exogenous Oxt expression was reduced approximately 50% by miR-24 compare to siControl transfection (error bars indicate standard deviations; *, p≤0.05 between control vector and miR-24 and between miR-24 and siControl).