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. Author manuscript; available in PMC: 2014 Jul 19.
Published in final edited form as: ACS Synth Biol. 2012 Nov 5;2(7):379–383. doi: 10.1021/sb3001062

Figure 3. Production of 36-methyl-FK506 via heterologous expression of the ibMCoA pathway in a S. sp. KCTC 11604BP ΔtcbB mutant strain.

Figure 3

Representative HPLC-ESI-MS chromatograms visualized by selected ion monitoring for FK506 (m/z 821 [M+NH4]+; in blue) and 36-Me-FK506 (m/z 835 [M+NH4]+; in red) in top panel. Results were obtained from five independent cultivation experiments of (a) the FK506 producing wild-type strain, (b) Streptomyces sp. KCTC 11604BP ΔtcbB, (c) the ΔtcbB mutant strain supplemented with 4-methylpentanoic acid (MPA), (d) the tcbB deletion strain with ermE*p-ibMCoA biosynthetic genes (pLD6), and (e) with the native promoter-ibMCoA biosynthetic genes (pMW1). Production levels of FK50 (in blue) and 36-Me-FK506 (in red) are graphically displayed in the bottom panel.