Table 2.

Comparison of infection reporter assay and the traditional crystal violet antiviral assay

	Crystal violet assay	VSV-DsRed2 assay
Antiviral incubation	24 hours, 67ul sample	24 hrs, 67ul sample
Infection	wt virus, 24 hour incubation	RFP reporter virus, 24 hour incubation
Fixation	4% PFA in 5% sucrose, 20 min
Rinse	2x PBS
Stain	Addition of crystal violet, overnight incubation
Scan	Microplate reader at 570 nm	Fluorescent microplate reader 485/620 or fluorescent scanner 555/580 nm.