Figure 1.

SDS-PAGE and Western blot analysis of total Lap (without supernatant fraction) L. monocytogenes isolates. Total protein was extracted from whole cells using sample solvent buffer and sonication (3 cycles of 20 min each). Each well was loaded with 5 μg protein in SDS-PAGE (4-15% polyacrylamide) gel. (a) Coomassie blue stained gel, (b) Corresponding Western blot immunoprobed with anti-Lap antibody (MAb-H7), Western blot of additional (c) clinical isolates and (d) food isolates. *F4244 is used as a positive control.