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. 2013 Jun 24;5:16. doi: 10.1186/1757-4749-5-16

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Copyright © 2013 Kim and Bhunia; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Transepithelial translocation characteristics of L. monocytogenes. (a and b) Transepithelial translocation characteristics of L. monocytogenes through paracellular route in Caco-2 cell monolayers by trans-well assays. (c) Dextran^FITC permeability through Caco-2 cell monolayer in trans-well model and (d) Transepithelial electrical resistance (TEER) in Caco-2 cell monolayers after treatment with high (H4, H8, H13) and low (CHLR4, CHLR6, ATCC 19117) Lap secreting isolates. L. monocytogenes F4244 (WT), lap mutant (lap-), lap complemented (lap+), ΔinlA and inlA complemented (inlA+) strains were used as controls. Data are average of at least three independent experiments performed in triplicate (n ≥ 9). Bars marked with different letters (a,b,c) are significantly different at P < 0.05.