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. 2013 Jul 2;12:65. doi: 10.1186/1475-2859-12-65

Figure 6.

Figure 6

Effect of rimP-SC disruption on MetK expression. (A) Detection of MetK by western blotting analysis. (B) Transcriptional analysis of metK by real-time RT-PCR. (C) The production of actinorhodin. Cell cultures (50 mg) at each time point were treated with KOH (final concentration, 1 N) and the OD640 corresponding to 10 mg of mycelium was determined. Data were presented as the averages of the results of three independent experiments in triplicate. Error bars showed standard deviations.