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. 2013 Jul 2;12:65. doi: 10.1186/1475-2859-12-65

Table 1.

Bacterial strains and plasmids used in this study

Bacterial strains/plasmids Relevant characteristics Source/references
Streptomyces
 
 
S. coelicolor M145
A derivative of the wild-type strain S. coelicolor A3(2) lacking plasmids SCP1 and SCP2
[6]
rimP-SCDM
The rimP-SC disruption mutant of S. coelicolor
This study
rimP-SCDMC
The complemented strain of rimP-SCDM
This study
M145/pNIK
S. coelicolor M145 containing the entire nikkomycin biosynthetic gene cluster
This study
rimP-SCDM/pNIK
rimP-SCDM containing the entire nikkomycin biosynthetic gene cluster
This study
M145/pPOL
S. coelicolor M145 containing the entire polyoxin biosynthetic gene cluster
This study
rimP-SCDM/pPOL
rimP-SCDM containing the entire polyoxin biosynthetic gene cluster
This study
S. venezuelae ATCC10712
The wild-type strain of S. venezuelae
[6]
rimP-SVDM
The rimP-SV disruption mutant of S. venezuelae
This study
rimP-SVDMC
The complemented strain of rimP-SVDM
This study
E. coli
 
 
JM109
recA1, endA1, gyrA96, thi-1, hsdR17, supE44, relA1, Δ(lac-proAB)/F’[traD36, proAB+, lacIq, lacZΔM15]
Invitrogen
BW25113
K-12 derivative; ΔaraBAD ΔrhaBAD
[32]
ET12567/pUZ8002
dam dcm hsdS cat tet/pUZ8002
[33]
rimPDM
The rimP disruption mutant of E. coli BW25113
This study
rimPDMC
The heterologous complemented strain of rimPDM
This study
rimPDM/pSET152::rrnFp::SCO5703
rimPDM containing pSET152::rrnFp::SCO5703 for heterologous complementation analysis
This study
BW25113/pSET152::rrnFp::xylE
BW25113 containing pSET152::rrnFp::xylE for catechol dioxygenase assay
This study
BW25113/pSET152::rrnFp::xylE*
BW25113 containing pSET152::rrnFp::xylE* for catechol dioxygenase assay
This study
rimPDM/pSET152::rrnFp::xylE
rimPDM containing pSET152::rrnFp::xylE for catechol dioxygenase assay
This study
rimPDM/pSET152::rrnFp::xylE*
rimPDM containing pSET152::rrnFp::xylE* for catechol dioxygenase assay
This study
Plasmids
 
 
pRIMPSC3
Plasmid used for the construction of rimP-SCDM
This study
pRIMPSC4
pSET152 containing the intact rimP-SC with its putative promoter
This study
pRIMPSV3
Plasmid used for the construction of rimP-SVDM
This study
pRIMPSV4
pSET152 containing the intact rimP-SV with its putative promoter
This study
pSET152::rrnFp::xylE
pSET152 containing the wild-type xylE and the promoter of rrnF for activity detection of catechol dioxygenase
This study
pSET152::rrnFp::xylE*
pSET152 containing the mutated xylE and the promoter of rrnF for activity detection of catechol dioxygenase
This study
pIJ10500::metK
pIJ10500 containing the intact metK with its promoter
This study
pIJ10500::sigR
pIJ10500 containing the intact sigR with its promoter
This study
pNIK
pSET152 containing the entire nikkomycin biosynthetic gene cluster
[34]
pPOL pSET152 containing the entire polyoxin biosynthetic gene cluster [35]