Table 1.
Bacterial strains and plasmids used in this study
Bacterial strains/plasmids | Relevant characteristics | Source/references |
---|---|---|
Streptomyces |
|
|
S. coelicolor M145 |
A derivative of the wild-type strain S. coelicolor A3(2) lacking plasmids SCP1 and SCP2 |
[6] |
rimP-SCDM |
The rimP-SC disruption mutant of S. coelicolor |
This study |
rimP-SCDMC |
The complemented strain of rimP-SCDM |
This study |
M145/pNIK |
S. coelicolor M145 containing the entire nikkomycin biosynthetic gene cluster |
This study |
rimP-SCDM/pNIK |
rimP-SCDM containing the entire nikkomycin biosynthetic gene cluster |
This study |
M145/pPOL |
S. coelicolor M145 containing the entire polyoxin biosynthetic gene cluster |
This study |
rimP-SCDM/pPOL |
rimP-SCDM containing the entire polyoxin biosynthetic gene cluster |
This study |
S. venezuelae ATCC10712 |
The wild-type strain of S. venezuelae |
[6] |
rimP-SVDM |
The rimP-SV disruption mutant of S. venezuelae |
This study |
rimP-SVDMC |
The complemented strain of rimP-SVDM |
This study |
E. coli |
|
|
JM109 |
recA1, endA1, gyrA96, thi-1, hsdR17, supE44, relA1, Δ(lac-proAB)/F’[traD36, proAB+, lacIq, lacZΔM15] |
Invitrogen |
BW25113 |
K-12 derivative; ΔaraBAD ΔrhaBAD |
[32] |
ET12567/pUZ8002 |
dam dcm hsdS cat tet/pUZ8002 |
[33] |
rimPDM |
The rimP disruption mutant of E. coli BW25113 |
This study |
rimPDMC |
The heterologous complemented strain of rimPDM |
This study |
rimPDM/pSET152::rrnFp::SCO5703 |
rimPDM containing pSET152::rrnFp::SCO5703 for heterologous complementation analysis |
This study |
BW25113/pSET152::rrnFp::xylE |
BW25113 containing pSET152::rrnFp::xylE for catechol dioxygenase assay |
This study |
BW25113/pSET152::rrnFp::xylE* |
BW25113 containing pSET152::rrnFp::xylE* for catechol dioxygenase assay |
This study |
rimPDM/pSET152::rrnFp::xylE |
rimPDM containing pSET152::rrnFp::xylE for catechol dioxygenase assay |
This study |
rimPDM/pSET152::rrnFp::xylE* |
rimPDM containing pSET152::rrnFp::xylE* for catechol dioxygenase assay |
This study |
Plasmids |
|
|
pRIMPSC3 |
Plasmid used for the construction of rimP-SCDM |
This study |
pRIMPSC4 |
pSET152 containing the intact rimP-SC with its putative promoter |
This study |
pRIMPSV3 |
Plasmid used for the construction of rimP-SVDM |
This study |
pRIMPSV4 |
pSET152 containing the intact rimP-SV with its putative promoter |
This study |
pSET152::rrnFp::xylE |
pSET152 containing the wild-type xylE and the promoter of rrnF for activity detection of catechol dioxygenase |
This study |
pSET152::rrnFp::xylE* |
pSET152 containing the mutated xylE and the promoter of rrnF for activity detection of catechol dioxygenase |
This study |
pIJ10500::metK |
pIJ10500 containing the intact metK with its promoter |
This study |
pIJ10500::sigR |
pIJ10500 containing the intact sigR with its promoter |
This study |
pNIK |
pSET152 containing the entire nikkomycin biosynthetic gene cluster |
[34] |
pPOL | pSET152 containing the entire polyoxin biosynthetic gene cluster | [35] |